Association of TNF-α, TNFRSF1A and TNFRSF1B gene polymorphisms with the risk of gastric cancer in Chinese population

Background: Gastric cancer (GC) is one of the most common malignancies worldwide. To date, the polymorphisms in the promoter region of tumour-necrosis factor-α (TNF-α) gene have been extensively studied in relation to different kinds of cancer, including GC. Methods and materials: In this case-control study, the case population consisted of 240 GC cases and 510 healthy controls. Genotyping of TNF-α, TNFRSF1A and TNFRSF1B polymorphism was determined by polymerase chain reaction restriction fragment length polymorphisms (PCRRFLP) analysis. Deviation of Hardy-Weinberg equilibrium was tested by using the χ2 test for goodness of fit. Results: A total of 240 GC patients and 510 healthy controls were enrolled in our study. In the present study, we evaluated the associations between the functional polymorphisms in TNF-α, TNFRSF1A and TNFRSF1B (rs1800629 and rs361525 in TNF-α sequences; rs767455, rs4149577 and rs1800693 in TNFRSF1A sequences and rs1061622 and rs1061624 in TNFRSF1B) and risk of GC. With respect to GC susceptibility, our data suggest that the TNFRSF1A rs767455 CT, TNFRSF1A rs4149577 CT and TNFRSF1A rs1800693 AG are risk factors of GC risk. However, the results of allele distribution of TNF-α, TNFRSF1A and TNFRSF1B SNP in cases and controls showed that no single allele was associated with the risk of GC. Conclusion: In conclusion, we found that the variant genotypes of rs4149577and rs1800693 may contribute to an increased risk of GC. Moreover, no allele was associated with the incidence of GC in Chinese Han population patients.